PUBLISHED RESEARCH
tRNA Regulation and Amino Acid Usage Bias in Plasmodium falciparum
We’re excited to share our latest collaborative work, published in iScience, and lead by Sherwin Chan’s team, where we dive into how Plasmodium falciparum adapts to its environment by regulating tRNA expression and amino acid (AA) usage. This work highlights a remarkable strategy the parasite uses to cope with nutrient availability, particularly when amino acids are scarce.
Single-cell transcriptomics reveal transcriptional programs underlying male and female cell fate during plasmodium falciparum gametocytogenesis
Thrilled to share our latest research in Nature Communications, where we performed single-cell analyses to computationally define new “driver genes” and study gene regulatory networks involved in male and female cell fate in Plasmodium falciparum gametocytes
Host-pathogen interactions in the Plasmodium-infected mouse liver at spatial and single-cell resolution
We present the first spatial map of malaria infection in the liver! In this study, we perform spatial transcriptomics in combination with single-nuclei RNA sequencing over multiple time points to delineate host-pathogen interactions across Plasmodium berghei-infected liver tissues
Single-Cell Transcriptomics To Define Plasmodium falciparum Stage Transition in the Mosquito Midgut
Malaria inflicts the highest rate of morbidity and mortality among the vector-borne diseases. The dramatic bottleneck of parasite numbers that occurs in the gut of the obligatory mosquito vector provides a promising target for novel control strategies.
Other publications:
Single-cell sequencing of tumor-associated macrophages in a Drosophila model
Dilan Khalili, Mubasher Mohammed, Martin Kunc, Martina Sindlerova, Johan Ankarklev and Ulrich Theopold.
Frontiers in Immunology. Volume 14 (2023). https://doi.org/10.3389/fimmu.2023.1243797
scDual-Seq of Toxoplasma gondii-infected mouse BMDCs reveals heterogeneity and differential infection dynamics
Franziska Hildebrandt, Mubasher Mohammed, Alexis Dziedziech, Amol K. Bhandage, Anna-Maria Divne, Fredrik Barrenäs, Antonio Barragan, Johan Henriksson and Johan Ankarklev.
Frontiers in Immunology. Volume 14 (2023). https://doi.org/10.3389/fimmu.2023.1224591
Single-Cell Transcriptomics To Define Plasmodium falciparum Stage Transition in the Mosquito Midgut
Mubasher Mohammed, Alexis Dziedziech, Vaishnovi Sekar, Medard Ernest, Thiago Luiz Alves E Silva, Balu Balan, S. Noushin Emami
Microbiology Spectrum. Volume 11, No. 2 (2023). https://doi.org/10.1128/spectrum.03671-22
Delineating Plasmodium liver infection across space and time
Franziska Hildebrandt, Johan Ankarklev, Kai Matuschewski.
Trends in Parasitology. Volume 39, Issue 2 (2023). https://doi.org/10.1016/j.pt.2022.12.005
Spatial Transcriptomics to define transcriptional patterns of zonation and structural components in the mouse liver
Nature Communications. Volume 12 (2021). https://doi.org/10.1038/s41467-021-27354-w
The spatiotemporal distribution of historical malaria cases in Sweden: a climatic perspective
Chen, Tzu Tung, Fredrik Charpentier Ljungqvist, Helene Castenbrandt, Franziska Hildebrandt, Mathias Mølbak Ingholt, Jenny C. Hesson, Johan Ankarklev, Kristina Seftigen, and Hans W. Linderholm.
Malaria Journal. 20, no. 1 (2021): 1-14
Generation of Transmission-Competent Human Malaria Parasites with Chromosomally-Integrated Fluorescent Reporters
McLean, K.J., Straimer, J., Hopp, C.S. et al.
Sci Rep 9, 13131 (2019). https://doi.org/10.1038/s41598-019-49348-x
Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria
Pierre-Yves Mantel, Daisy Hjelmqvist, Michael Walch, Solange Kharoubi-Hess, Sandra Nilsson, Deepali Ravel, Marina Ribeiro, Christof Grüring, Siyuan Ma, Prasad Padmanabhan, Alexander Trachtenberg, Johan Ankarklev, Nicolas Brancucci, Curtis Huttenhower, Manoj Duraisingh, Ionita Ghiran, Winston Kuo, Louis Filguieira, Roberta Martinelli, and Matthias Marti
Nature Communications. 2016 Oct 10;7:12727. doi: 10.1038/ncomms12727
Sex: how malaria parasites get turned on
Ankarklev J, Brancucci NM, Goldowitz I, Mantel PY, Marti M
Curr Biol. 2014 May 5;24(9):R368-70. doi: 10.1016/j.cub2014.03.046
Simultaneous transcription of duplicated var2csa gene copies in individual Plasmodium falciparum parasites
Brolin KJ, Ribacke U, Nilsson S, Ankarklev J, Moll K, Wahlgren M, Chen Q.
Genome Biol. 2009;10(10):R117.
Comparative analyses of freshly isolated Giardia intestinalis assemblage A isolates
Ankarklev J, Franzén O, Peirasmaki D, Jerlström-Hultqvist J, Lebbad M, Andersson J, Andersson B, Svärd S
BMC Genomics. 2015 Sep 15;16(1):697. Doi: 10.1186/s12864-015-1893-6
Allelic sequence heterozygosity in single Giardia parasites
Johan Ankarklev, Staffan Svärd, Marianne Lebbad,
BMC Microbiol. 2012 May 3;12:65
Behind the smile: cell biology and disease mechanisms of Giardia species
Johan Ankarklev, Jon Jerlström-Hultqvist, Emma Ringqvist, Karin Troell and Staffan Svärd
Nature Rev Micro 2010 Jun;8(6):413-22
